A famous grizzly bear beloved for decades by countless tourists, biologists and professional wildlife photographers in Grand Teton National Park is dead after being struck by a vehicle in western Wyoming.

Grizzly bear 399 was killed on Tuesday after a vehicle hit it on U.S. Highway 26/89 in Snake River Canyon, south of Jackson, Wyoming. The bear’s identity was confirmed through ear tags and a microchip.

Grizzly bear 399, a legendary sow nearly 30 years in age who had nearly two dozen cubs over the years, was killed in a vehicle collision in the Snake River Canyon south of Jackson, Wyoming.